Supplies: Fibroblast Biopsy Transport Media (T115)Ĭontainer/Tube: Sterile container with any standard cell culture media (eg, minimal essential media, RPMI 1640). An additional 3 to 4 weeks is required to culture fibroblasts before genetic testing can occur. A separate culture charge will be assessed under CULFB / Fibroblast Culture for Biochemical or Molecular Testing. Specimen Stability Information: Ambient (preferred)/Refrigerated <24 hoursĪdditional Information: Indicate the tests to be performed on the fibroblast culture cells.
#Scid syndrome full#
Specimen Volume: 1 Full T-75 or 2 full T-25 flasks
Specimen Type: Peripheral blood mononuclear cells (PBMC)Ĭollection Instructions: Send as a suspension in freezing medium or cell pellet frozen on dry ice. For collection instructions in Chinese, see Blood Spot Collection Card-Chinese Instructions (T800) For collection instructions in Spanish, see Blood Spot Collection Card-Spanish Instructions (T777)Ĥ. For collection instructions, see Blood Spot Collection Instructionsģ. Due to lower concentration of DNA yielded from blood spot, it is possible that additional specimen may be required to complete testing.Ģ. Specimen Stability Information: Ambient (preferred)/Refrigeratedġ. Do not expose specimen to heat or direct sunlight. Let blood dry on the filter paper at ambient temperature in a horizontal position for a minimum of 3 hours.3.
#Scid syndrome how to#
See Dried Blood Spot Collection Tutorial for how to collect blood spots via fingerstick.Ģ. An alternative blood collection option for a patient older than 1 year of age is fingerstick. Preferred: Collection card (Whatman Protein Saver 903 Paper)Īcceptable: Whatman FTA Classic paper, PerkinElmer 226 (formerly Ahlstrom 226) filter paper, or blood spot collection cardġ. Supplies: Card-Blood Spot Collection Filter Paper (T493) Specimen Stability Information: Ambient (preferred) 4 days/Refrigerated 14 days Send whole blood specimen in original tube. Submit only 1 of the following specimens:Ģ. Call 80 for instructions for testing patients who have received a bone marrow transplant. Patient Preparation: A previous bone marrow transplant from an allogenic donor or a recent (ie, <6 weeks from time of sample collection) heterologous blood transfusion will interfere with testing.
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